Part:BBa_K4221031

RGD-GSlinker

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage

Near-infrared (NIR) fluorescent probes have become powerful tools for non-invasive monitoring of various biologically important processes in vivo. To meet the requirement of improving the function of NIR, one has to find a material that can render NIR fluorescent probes soluble and selective simultaneously.the hybrid hydrophobin-RGD fusion protein preserving dual abilities can render NIR fluorescent probes soluble and selective simultaneously. Our team is trying to improve self-assembly and targeting functions from HFBI and RGD peptideand replacing HFBI with BslA.[2]

Biology

RGD is a tripeptide sequence containing arginine-glycine-aspartate, which is a recognition site for the interaction between integrins and their ligands. It mediates the adhesion between cells and extracellular matrix and between cells, and has signal transduction function, thereby mediating many important life activities.[1]

Design Consideration

The construction includes:

RGD is fused with a GS linker（GGTGGTGGCGGCAGCGGCGGAGGCGGTAGT）

Reference

[1]Qu Hong, WangXiangcheng, Wang Cheng, etal. Research progress and clinical application of RGD peptide[J]. Journal Of Inner Mongolia Medical University, 2019, 41(6):660-663.

[2]Xiao Y, Zhang Q, Wang Y. Dual-functional protein for one-step production of a soluble and targeted fluorescent dye[J]. Theranostics, 2018, 8(11):3111-3125.